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Non Hodgkin lymphoma (NHL)Only 14 pages are availabe for public view
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Abstract
Lymphoma describes a group of blood cancers that develop in the lymphatic system and affecting lymphocytes. Human chemokines are low molecular weight proteins released by different cell types. SDF1 is also known as CXCL12 and it exert its effects via binding to GPCR (CXCR4). The stromal cell-derived factor-1 (SDF-1) gene contains a common polymorphism named rs1801157 (G801A). Studies found an association between this polymorphism and many diseases as lung cancer, breast cancer, acute myeloid leukemia, lymphomas and viral infections such as acute hepatitis. Aim: The aim of this work was to study the CXCL12 gene polymorphism by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assay and its association with NHL. Setting: Oncology Center Mansoura University (OCMU). Results: NHL cases were classified into 92% B cell lymphoma and 8% T cell lymphoma. Studied NHL cases were diagnosed in different stages, 9% of cases in stage I, 35% in stage II, 33% in stage III and 23% cases in stage IV. Bulky disease was diagnosed in 9% of the cases. There was significant decrease in albumin, bilirubin, while significant increase in AST, ALT, uric acid and LDH in NHL when compared to control subjects. Clinical outcome of the studied cases was as follow: 67% of cases achieved complete remission (CR), 30% achieved partial remission (PR) and 3% were stable disease (SD). Fifteen % of the patients died during the study. As regard SDF-1 genotyping 59% of NHL patients had a (GG) alleles genotype while 41% had an (A) allele genotype (34% were heterozygous A/G and 7 % were homozygous A/A ), while among the control group 58% had a (GG) alleles genotype and 42% had an (A) allele genotype (39% were heterozygous A/G and 3% were homozygous A/A). There is no significant difference in the risk of developing NHL between patients and controls. Comparison between CXCL12 (G/G) alleles genotype and (A) allele (A/G & A/A) genotype regarding age, gender, hematological laboratory data and clinical chemistry data showed no significant differences between control and NHL patients. SDF rs1801157 genotypes showed significant difference in stable disease (SD) (p=0.034), this significance was attributed to significantly higher frequency of SD in patients carrying AA genotype when compared to those carrying GG genotypes (p=0.003). Otherwise, no significant differences were found between different genotypes regarding CR, PR and total mortality rate in studied NHL group. As regard survival, no significant differences were found in OS and DFS between G/G, A/G and A/A alleles. Conclusion : The stromal cell derived factor-1 gene polymorphism could be implicated in susceptibility to NHL. However, in the present study no significant association was observed between SDF-1 gene polymorphism and NHL risk, survival and prognosis.