الفهرس 
Respiratory syncytial virus (RSV)Only 14 pages are availabe for public view
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Abstract
In this study, the incidence of RSV among infants and young children
attending Menoufia University Hospital during the winter season was detected
using two diagnostic methods tissue culture and RT-PCR.
Nasopharyngeal aspirates (NPAs) were collected from Ninety seven
patients in Pediatric Department of Menoufia University Hospital in two
seasons; from October 2013 to April 2014 and from October 2014 to April
2015. Patients were evaluated for symptoms of acute respiratory tract
infection (nasal congestion, sore throat, cough, sputum production, fever or
earache).
The age distribution among the cases of LRTIs in this study illustrated
that about 72% of the cases were less than one year of age. The highest
percentage of cases (45.4%) was seen in the age group from 0-6 months,
followed by the age group from 6-12 months (26.8%), age group from 12-18
months (16.5% ), age group from 18-24 months (6.2%) and age group >24
months up to 5 years (4.1%). The mean age for the selected cases was
9.32.31 months (range 1.3-to >24 months up to 5 years). There was
predominance of males among patients of LRTI (69.1%).
All NPAs were tested for the presence of RSV by tissue culture and RTPCR.
There was a highly significant difference between two previous
methods for detection of RSV (P value =0.0001). Thirty out of 97 (30.9%)
from NPAs showed RSV- like CPE (syncytia formation) in HEP-2 cells, while
68.04% of NPAs (66 specimens) were positive for RSV by RT-PCR.