الفهرس 
Mesenchymal stem cellsOnly 14 pages are availabe for public view
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Abstract
Mesenchyme stem cells (MSCs) defined as a population of progenitor cells capable of giving rises to at least three mesodermal lineages in vitro, the chondrocytes, osteoblasts and adipocytes. MSCs have the advantages of easy isolation and cultivation, expansion potential, stable phenotype, compatibility by different delivery methods and minor side effects after implantation.
MSCs are used in regenerative medicine mainly. In addition, MSCs hold immune modulatory properties represented by cell to cell contact and by secretion of immune regulatory molecules. Thus, MSCs represent promising candidates for the prevention and treatment of immune-mediated diseases e.g, rheumatoid arthritis.
The aim of this study is to evaluate the immune modulatory effect of human mesenchymal stem cells on T lymphocytes of rheumatoid arthritis patients.
The present study involved 30 patients of rheumatoid arthritis, it was held at clinical pathology department, EL Menofyia University Hospital during the period from May 2014 to March 2015.
Patients were subjected to the following:
1. History taking: name, age, sex, complaint taken by the patient own words laying stress on disease duration and pain with evaluation of its severity.
2. Clinical examination
3. Laboratory investigations: CRP, ESR, RF and ACCP
In this study, 10 ml was aspirated from normocellular bone marrow donors under aseptic technique. Mononuclear cells were separated by density gradient centrifugation and cultured in 25cm2 flasks for isolation, purification and expansion of MSCs.
5cm peripheral blood was obtained from rheumatoid arthritis patients, the tubes were centrifuged and the mononuclear cells fraction were collected. The MSCs were co-cultured with MNCs from rheumatoid arthritis. The sample groups were divided into the following groups: group 1: Peripheral blood mononuclear cells culture without PHA (-ve control), group 2: Peripheral blood mononuclear cells culture with PHA and group 3: Peripheral blood mononuclear culture with PHA and mesenchymal stem cells.
After 72 hours of the culture, the cells were harvested and analyzed by flowcytometey, culture supernatant was aspirated from each well and was stored at -20°c for further measurement of IL17 by ELISA.
When compared between group 1 and group 2, there is clinical significant difference in percentage of CD4+ and CD8+ T lymphocytes and supernatant IL17 being higher in group 2.
When compared between group 2 and group 3, there is clinical significant difference in percentage of CD4+ and CD8+ T lymphocytes and supernatant IL17 being lower in group 3.
There is also significant indirect correlation between patient ACCP and CD4+ T lymphocytes cultured with MSCs.
The present study proved the possibility of in vitro immunosuppression effect of bone marrow derived MSCs on T lymphocytes of rheumatoid arthritis patients thus we hope to be a new safe tool in the management of the disease.