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Abstract Two field experiments were carried out in the Experimental Farm , of the Faculty of Agriculture , Fayoum University on Sandy Loam soil during 2011 / 2012 and 2012 / 2013 seasons to study the effects of two organic manure levels i.e. ( 15 and 30 m3 / fed. ), bio-fertilizer (Azotobacter who was inoculation the seeds) and three levels of nitrogen fertilizer .[ 100% ( 75 kg N / fed. ) & 50 % ( 37.5 kg N / fed. ) and 25 % ( 18.75 kg N / fed. ) ] of Urea ( 46 % N )on two wheat cultivars i.e. ( sakha 93 ( v1 ) &Sakha 94 ( v2 ) ).The treatments were set in Randomized Complete Block Design ( RCBD ) in factorial arrangement with three replications . Significant effect for wheat varieties were obtained on plant height ( cm ) , spike length ( cm ) and straw yield ( ton / fed. ) in the 1st season and on total protein percentage in both seasons but was insignificantly on number of tillers / plant , number of spikes / plant , number of grains / spike , weight of grains / spike( g ) , grain yield (ton / fed. ) , 1000-grains weight ( g ) and harvest index ( % ) in both seasons .The superiority was for Sakha 94 in most characters . Fertilizer treatments was significantly effect on plant height ( cm ) , number of tillers / plant , number of spikes / plant and 1000- grains weight ( g ) in 1st season , on spike length ( cm ) and harvest index ( % ) in 2nd season and on number of grains / spike , weight of grains / spike ( g ) , grain yield ( ton / fed. ) straw yield ( ton /fed. ) and total protein percentage in both seasons .F3 ( 30 m 3 / fed. organic manure + bio – fertilizer + 50 % ( 37.5 kg N / fed. )) treatment gave the highest values followed by F4 ( 30 m 3 / fed. organic manure + bio – fertilizer + 25 % ( 18.75 kg N / fed.)) or F5 ( 30 m3 / fed. organic manure + 50 % ( 37.5 kg N / fed. )) treatments . Interaction between varieties and fertilizer treatments was significantly effect on number of tillers / plant , number of spikes / plant , weight of grains / spike and straw yield ( ton /fed. ) in both seasons , on number of grains / spike in 2nd season and on grain yield ( ton / fed. ) and total protein ( % ) in 1st but was insignificantly on plant height ( cm ) , spike length ( cm ) , 1000 – grains weight ( g ) and harvest index ( % ) in both seasons . Finally , by using organic fertilizer and / or bio-fertilizer with half dose of mineral fertilizer it could be plant growth and yield . Besides , using bio-fertilizers that contain different microbial strains had led to decrease in the use of chemical fertilizers and had provided height products free of harmful agrochemicals for human safety. Key word : Wheat ( Triticum aestivum, L. ) , Organic manure , Bio-fertilizer , Nitrogen fertilizer , Varieties . |