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Abstract Breast cancer is the most common cancer and a leading cause of death among women. Breast cancer screening plays an important role in early detection and decreasing mortality rate. The aim of this study was to evaluate effect of health education program on women knowledge, attitudes, and behaviors regarding breast cancer screening in health care centers in Port Said city. This study was carried out in two primary health care centers (Omar ben Elkatab and Elkuwait health center) .Convenience sample of 210 women who attended family planning clinics .Data were collected through using four tools: structured interviewing questionnaire sheet, health belief model scale, breast Cancer Worry Scale and observational checklist for breast self- examination practice. The study results revealed that there was statically significant improvement of women knowledge, attitudes and practice regarding breast cancer and its screening practices after implementing the program, also there was statistically significant positive correlation between women’s knowledge, attitude, practice of breast self-examination and education. Based on the result of the study it was recommended, continuing need for more BC educational programs that are intended to attract the attention of women in reproductive age, breast cancer and its screening should be integrated in preventive health programs in primary health care centers and further research on women false belief that hinder using of breast cancer screening services |