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Abstract The Nile River often thought of as the backbone of Egypt, has been source of great sustenance and mobility throughout the years. The heavy-metals are carried intostreams, rivers, lakes and oceans and may be deposited in sediments at the bottom of the water body or they may evaporate and be carried elsewhere as rainwater. In our study hundred water samples were collected from River Nile in Rod El-Farag region,Cairo, Egypt. to make isolation and identification of some bacterial strains that have the ability to bear and resist high concentrations of Al, Fe and Mn metals up to 5 mg/l. The isolated bacterial strains were Pseudomonas putida biotype A, Citrobacterfreundiiand Aeromonashydrophila DNA Group1.The first and second strains had the ability to resist up to 5 mg/l Fe, Mn and Al separately; while the last one had the ability to resist up to 5 mg/l Al only. The strains were identified by IMVIC test andconfirmatory tests were done by Biolog GEN III Microplate technique.Thewater samples were supplemented with 2 mg/l of Fe, 2 mg/l of Mn and 2 mg/l Al metal ions together in their salt form FeCl3.6 (H2O), MnSO4.H2O and AlKO8S2.12 (H2O) respectively as a spike sample the pH became 7.2. The values of Fe, Mn and Al of raw water before addition were 0.450 mg/l, 0.075 mg/l and 0.352 mg/l respectively. The values of Fe, Mn and Al in spike sample were 2.493 mg/l, 2.099 mg/l and 2.405 mg/l respectively. The amount of dead bacterial biomass added was (15 mg / 150 ml) and the temperature was 280C.In our experiment we fixed some parameters liketemperature, pH and amount of dead microbial biomass added while the contact time was the variable factor in our experiment beside the type of metals. Pseudomonas putida dead biomass had the ability to biosorb Fe, Mn and Al metals in ionized form from water. |