الفهرس | Only 14 pages are availabe for public view |
Abstract This study aimed to determine the concentration of some estrogens like 17β-estradiol, 17α-ethynylestradiol and bisphenol A in agriculture drainage water that used in Egyptian fish farms and tap water also to evaluate the oxidative degradation effect of potassium permanganate on these estrogenic pollutants. In addition to assess vitellogenin gene expression and GSI on mature male Oreochromis niloticus as a biomarker for estrogenisity. The concentrations of E2, EE2 and BPA assessed by High Performance Liquid chromatography (HPLC) were 9.150, 16.655 and 0.371 mg/L respectively in ponds water and only 0.125 mg/L bisphenol in tap water. This concentration declined in agriculture water treated by 2.5 ppm, moreover E2 and EE2 were completely eliminated with 5 ppm. As an accurate biomarker for estrogenicity, vitellogenin gene expression was assessed in the livers of all groups. A significant downregulation in farm treated groups was observed compared to control, it was 9.25 and 34.48 fold for 2.5 and 5 ppm, respectively and in tap water 0.90 fold and 1.97 fold for 2.5 and 5 ppm, respectively. In farm treated groups GSI values were higher than the control group almost by the double. |