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العنوان
Oxidative Stress As A Mechanism Of Cadmium Induced Hepatotoxicity And Renal Toxicity And Protection By Zinc =
المؤلف
Elshenawy, Wesam Ahmed.
هيئة الاعداد
باحث / Wesam Ahmed Elshenawy
مشرف / Ahmed Sobhey El-sharaky
مشرف / Al-sayeda Al-sayed
مشرف / Nihal Mohamed Elguindy
مشرف / Heba Mohammad Abdou
الموضوع
Renal toxicity. Hepatotoxicity.
تاريخ النشر
2015
عدد الصفحات
160 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الكيمياء الحيوية ، علم الوراثة والبيولوجيا الجزيئية
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة الاسكندريه - كلية العلوم - Biochemistry
الفهرس
Only 14 pages are availabe for public view

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Abstract

Liver and kidney from all experimental animals were immediately isolated, cleaned from blood adhering matters, washed in ice-cold saline, and dried. Parts from the liver and kidney of each group were sliced and immediately fixed in 10% formalin for histological examination. One fourth gram from each liver tissue was homogenized separately in 2 ml cold buffer (50mM potassium phosphate pH 7.5, 1mM EDTA) per gram tissue using tissue homogenizer (Tekmar model TR-10, West Germany). The homogenate was centrifuged at 4000 rpm for 15 minutes using cooling centrifuge (Hettich model EBA 12R, Germany). Then, the supernatants were stored at -80°C for reduced glutathione (GSH) determination. The remaining of liver and kidney tissues were frozen at -20°C for the other biochemical investigations.