الفهرس 
Hepatitis C Virus Infection
For TNF-?-308 gene polymorphismOnly 14 pages are availabe for public view
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Abstract
Hepatitis C virus infection affects more than 170 million people worldwide;Patients with HCV may develop chronic infection that may result in liver cirrhosis, hepatic failure or HCC.
A number of viruses and host genetic factors may affect the risk for development of HCC mediated by HCV, one of host genetic parameter is genetic polymorphism
The detection of genetic polymorphism within promoter regions of cytokine gene sequences as TNF-α, and TGF-β1 may be associated with different levels of gene transcription which will affect the development of HCC in patientswith chronic HCV.
This study aimed to find out the association between TNF-α-308 and TGF-β1-509 gene polymorphisms with HCC in chronic HCV infected patients.
Fifty-two subjects (32 patients diagnosed as HCC on top of chronic HCV infection and 20 apparently healthy controls) were included in this study.
All subjects included in the study were investigated for TNF-α-308 and TGF-β1-509polymorphism by PCR-RFLP technique.
The frequency of CC TGF-β1- 509(the wild genotype) was 15.6% while TT and CT (the polymorphic genotypes) were 31.3% and 53.1%, respectively among patients.The T allele was significantly higher in patients group (57.8%) compared to control group(35%).
A statistically significant association between TGF-β1-509 gene polymorphism and HCC was detected.
An association between higher T allele frequency of TGF-β1-509 and risk of HCC in chronic HCV patients was detected.
The frequency of GG TNF-α-308 genotype (the wild genotype) was 37.5% while AA and GA (the polymorphic genotypes) were 15.6% and 46.9%, respectively among patients and there was no statistical significant difference between the two studied groups regarding the allelic distriution.
The association between TNF-α- 308 gene polymorphism and HCC could not be proved.
No statistically significant difference of serum AFP among the different genotypes of either TNF-α- 308 or TGF- β1-509 genes was detected.