الفهرس 
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Abstract
A total of 600 serum samples were collected from different farm animals to be screened serologically by different serological tests for presence of Brucella antibodies for a period of 12 months from June 2014 to May 2015 including; cattle (200), buffaloes (100), sheep (200) and goats (100). In addition, 300 human serum samples were collected throughout the year. Serum samples were examined by RBPT, i. ELISA and c. ELISA to estimate the prevalence of brucellosis in farm animals and humans in Alexandria Province and to investigate the epidemiological pattern of such disease among humans and animals.
The obtained results could be summarized in the following points:
• The overall seroprevalence of brucellosis in farm animals in the current study by different serological tests including RBPT, i. ELISA and c. ELISA was 11, 6.67 and 5.67 %, respectively.
• The seroprevalence of brucellosis in cattle was 10, 7 and 6% by RBPT, i. ELISA and c. ELISA, respectively.
• The seroprevalence of brucellosis in buffaloes was 12, 8 and 8% by RBPT, i. ELISA and c. ELISA, respectively.
• The seroprevalence of brucellosis in sheep was 13, 8 and 6% by RBPT, i. ELISA and c. ELISA, respectively.
• The seroprevalence of brucellosis in goats was 8, 3 and 2% by RBPT, i. ELISA and c. ELISA, respectively.
• Chi square analysis of the obtained results showed significant relationship between animal species and the prevalence of brucellosis.
• Sex based seroprevalence of brucellosis in cattle depending on the results of RBPT revealed that the prevalence was 11.03 and 7.81% in females and males, respectively.
• Sex based seroprevalence of brucellosis in buffaloes depending on the results of RBPT revealed that the prevalence was 12.16 and 11.54% in females and males, respectively.
• Sex based seroprevalence of brucellosis in sheep depending on the results of RBPT revealed that the prevalence was 11.45 and 20.59 % in females and males, respectively.
• Sex based seroprevalence of brucellosis in goats depending on the results of RBPT revealed that the prevalence was 9.64 % in females while all samples of males were found to be negative.
• Chi square analysis of the obtained results showed significant relationship between sex and the prevalence of brucellosis in the examined animal species except buffaloes.
• Age based seroprevalence of brucellosis in cattle depending on the results of RBPT revealed that the highest seroprevalence was observed in the age group (≥14 years) (16.30%) followed by the age group (7- ˂14 years) (11.84%) and finally the age group (1- ˂7 years) (6.45%).
• Age based seroprevalence of brucellosis in buffaloes depending on the results of RBPT revealed that the highest seroprevalence was observed in the age group (≥10 years) (20.0%) followed by the age group (5- ˂10 years) (13.98%) and finally the age group (1- ˂5 years) (9.26%).
• Age based seroprevalence of brucellosis in sheep depending on the results of RBPT revealed that the highest seroprevalence was observed in the age group (≥3 years) (18.75%) followed by (2- ˂ 3 years) (17.78%) then the age group (1- ˂ 2 years) (17.24%) and finally the age group (˂ 1year) (3.28%).
• Age based seroprevalence of brucellosis in goats depending on the results of RBPT revealed that the highest seroprevalence was observed in the age group (2- ˂ 3 years) (12.90%) followed by the age group (1- ˂ 2 years) (11.11%) then the age group (˂ 1year) (3.03%) and finally the age group (≥3 years) (0%).
• Chi square analysis of the obtained results showed significant relationship between age groups and the prevalence of brucellosis in the examined animal species.
• Seroprevalence of brucellosis in cattle depending on the results of RBPT in relation to locality in Alexandria province revealed that the highest prevalence was observed in El- Aameria and Borg El Arab (12% of each) followed by El Montazah (10%) then Abis (9%).
• Seroprevalence of brucellosis in buffaloes depending on the results of RBPT in relation to locality in Alexandria province revealed that the highest prevalence was observed in Borg El Arab (12.5%) followed by Abis (11.36%) then El Montazah (9.68%) and finally El- Aameria (1.76%).
• Seroprevalence of brucellosis in sheep depending on the results of RBPT in relation to locality in Alexandria province revealed that the highest prevalence was observed in El Montazah (16%) followed by El- Aameria and Borg El Arab (13.33% of each) then Abis (8%).
• Seroprevalence of brucellosis in goats depending on the results of RBPT in relation to locality in Alexandria province revealed that the highest prevalence was observed in in El- Aameria and Borg El Arab (11.43% of each). On contrary, examined serum samples of El Montazah and Abis were found to be negative.
• Statistical analysis of the results showed significant association between the seroprevalence of brucellosis and locality in all examined animal species except cattle.
• The overall seroprevalence of brucellosis among examined 300 human serum samples in Alexandria province through RBPT, i. ELISA and c. ELISA was 14.67, 10.33 and 11.67%, respectively.
• Statistical analysis showed that there was a significant association at (P<0.0001) between the results of RBPT, i. ELISA and c. ELISA.
• Seroprevalence of brucellosis among examined human beings depending on the results of RBPT in relation to place of residence in Alexandria province showed that the highest prevalence was noticed in El- Aameria (18.40%) followed by Gharb (17.86%), Borg El Arab (16.66%), Sharq (13.33%), El Montazah (13.24%) and Wasat (12.5%). On contrary, all the examined samples that were collected from El- Gomrok were found to be negative.
• Statistical analysis showed that there was a significant association at (P<0.0001) between the seroprevalence of brucellosis among examined human beings in relation to place of residence in Alexandria province.
• Seroprevalence of brucellosis among examined human beings depending on results of RBPT in relation to their health status clarified that 25% of the examined samples collected from patients complaining of fever were found to be positive for brucellosis. On the other side, only 7.78% of the examined samples of apparently healthy individuals were found to be positive for brucellosis with significant association.
• Seroprevalence of brucellosis among examined human beings depending on the results of RBPT in relation to sex showed that males’ prevalence (16.67%) was higher than that in females (12.12%) with significant association.
• Seroprevalence of brucellosis among examined human beings depending on the results of RBPT in relation to age groups showed that the highest prevalence was observed in the age group (20 - ˂ 40 years) (19.75%) followed by the age group (˂ 20 years) (13.48%) then the age group (40 - ˂ 60 years) (12.79%) and lastly the age group (˃ 60 years) (11.36%) with non-significant association between different age groups and prevalence of human brucellosis with significant association.
• Seroprevalence of brucellosis among examined human beings depending on the results of RBPT in relation to location illustrated that the seroprevalence of brucellosis was higher in those inhabiting urban areas (17.08%) than in those inhabiting rural areas (15.54%) with non-significant association between location and prevalence of human brucellosis.
• Seroprevalence of brucellosis among examined human beings depending on the results of RBPT in relation to occupation showed that the highest seroprevalence was observed in farmers (16.30%) followed by housewives (14.25%), animals’ attendants (14.10%), veterinarians (11.11%), slaughterhouse workers (8.33%) and finally veterinary students (0.59%) with significant association between different occupations and prevalence of human brucellosis.
• Seroprevalence of brucellosis among examined human beings depending on the results of RBPT in relation to milk consumption. It was found that the highest seroprevalence was found in those who irregularly drinking milk (15.97%), followed by those who rarely drinking milk (13.88%) and lastly those who drink milk regularly (13.33%) with non-significant association between milk consumption and prevalence of human brucellosis.
• It was found that the seroprevalence of brucellosis in the examined samples of humans was higher in those who were not in direct contact with farm animals (16.05%) than those who were in direct contact with farm animals (14.15%) with non-significant association between type of animalcontact and.