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Abstract The most tolerant variety (Giza 168) and the most sensitive one (Gemmeiza 9) were selected according to the drought susceptibility index (DSI).. The two selected varieties, their F1 and F2 plants were evaluated for their response to drought stress by recording some drought - related traits in a completely randomized design experiment under drought stress (150 mm water), the data were recorded for all plants (parents,F1 and F2 plants) . The F2 plants were represented by 151 plants and classified into groups according to the obtained data for above-mentioned traits depending to their behavior under drought stress to detect the most tolerant five F2 plants and the most five sensitive F2 plants. To determine if traits data normally distributed, the skewness and kurtosis values were calculated for each trait .The program Windows QTL Cartographer V.2.5 A total of 121 QTL with a significance ranging from 5% to 1% were identified for nine traits. These included: 15 QTL for plant height, 18 QTL for flag leaf area, 7 QTL for the number of leaves, 16 QTL for a number of tillers, 16 QTL for spike length, 16 QTL for a number of spikelets /spike, 7 QTL for biological yield, 11 QTL for grain yield and 15 QTL for number of kernels/spike. The results indicated the presence of two positive and four negative markers using seven RAPD primers. Five ISSR primers exhibited four positive and one negative markers. These markers could be considered as reliable markers for drought tolerance in bread wheat. |