الفهرس 
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Abstract
The term “alkaliphiles” is used for microorganisms that grow optimally or very well at pH values above 9. Alkaliphiles consist of two main physiological groups of microorganisms; alkaliphiles and haloalkaliphiles. Alkaliphilies require an alkaline pH of 9 or more for their growth and have an optimal growth at pH around 10, whereas haloalkaliphilies require both an alkaline pH ( 9) and high salinity (up to 33% NaCl, wt/vol.). Extremely alkaline lakes and deserts for example, Lake Magadi in Kenya and Wadi El-Natrun in Egypt, are probably the most stable highly alkaline environments on Earth, with a consistent pH of 10.0 to 12.0 depending on the site.
Studies of alkaliphilie have led to the discovery of many types of enzymes that exhibit interesting properties. Some of these enzymes such as alkaline proteases, xylanases, celluloses, pectinases and chitinases have been produced on an industrial scale.
The main objectives of this investigation include:
(1) Isolation and identification of some haloalkaliphilic bacterial isolates from Wadi El-Natrun.
(2) Studying the ability of these isolates to produce useful enzymes.
Plan of Work
1. Isolation of some haloalkaliphilic bacilli from Wadi El-Natrun.
2. Screening for these isolates’ potential to express extracellular enzymes.
3. selection of the most promising isolate(s), capable of producing remarkable enzyme(s).
4. Optimization of the physiological conditions for the production of enzyme(s).
5. Identification and molecular characterization of the isolate(s.