الفهرس 
1. INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Summary
185
8. Summary
This study was conducted to establish a protocol for the callus production
from S. marianum plant and best conditions of the high yield production of medical constituents. Important results of medium optimization
1. S. marianum callus FW recorded the highest accumulation with medium K
after 21 d. Low values of callus FWwere in media E and H with 26 mg and
28 mg respectively after 21d. After 42 and 63 d medium F recorded the
highest callus mass production.
2. Medium K produced the highest accumulation of callus DW after 21 d.
While medium F produced the highest amount 45 and 118 mg after 42 and
63 d respectively. Medium Hand D produce the lowest amount of callus dry
weight after 21, 42 and 63 d respectively.
3. The callus growth rate exhibited a decreasing trend of variation with time of
callus growth. Growth rate of callus recorded the highest percentage 58 % in
media Kand 12 % in media F after 21 and 42 d respectively. While media D
produced the lowest percentage of callus growth rate.
4. The mediumI, G and Fproduced the maximum volume of callus after 21, 42
and 63 d respectively. Media B, D and H recorded the lowest volume of
callus after 21, 42 and 63 d respectively.
5. Medium F at 63 day led to the highest percentage of callus water content
1225 %. Medium H led to the lowest callus water contents in the range of
500 % after 63 day. Medium Mand L caused high amount of callus water
content after 21and 42 d respectively.