الفهرس 
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Abstract
Summary
SCC is considered the most common epithelial malignant disease arising from the mucosa of the upper aerodigestive. The disease is characterized by local tumor aggressiveness, early recurrence and high frequency of second primary tumors.
In this study, the effect of MSCs on HEp2 cells was evaluated regarding the expression of Survivin, Bcl2 and VEGF as well as proliferation rates.
The material of this study consisted of five groups. The first group consisted of HEp2 culture that acts as a control. The second and third groups consisted of HEp2 cells cultured in MSCs conditioned medium for 48 and 96 hours, respectively. The fourth and fifth groups consisted of HEp2 cells cultured in coculture conditioned medium for 48 and 96 hours, respectively. The above mentioned cultures were evaluated for Survivin, Bcl2 and VEGF gene expression and for proliferation rates. The expression of the three genes was evaluated using real time PCR and the proliferation rate was evaluated using MTT proliferation assay.
The results of this study showed that culturing HEp2 in MSCs conditioned media and in coculture conditioned media for 48 hours reduced Survivin, Bcl2 and VEGF gene expressions when compared to their expression in the HEp2 culture as well as proliferation rates. There was a further reduction in expression in Survivin and VEGF gene expressions and proliferation rates when culturing in these media was extended to 96 hours.
Bcl2 gene expression, nevertheless, increased significantly after 96 hours of culturing in MSCs and co-culture conditioned media when compared to its expression in these media after 48 hours. It rose in the HEp2 cell line in co-cultured medium after 96 hours to such an extent that it even exceeded its expression in the HEp2 culture.
from this study, it could be deducted that MSCs produce soluble mediators (when cultured alone or when cultured with HEp2 cells) that resulted in reduced proliferation and angiogenesis of HEp2 cells. where as the effect of these mediators on apoptosis needs further studying before its application clinically.