الفهرس 
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Abstract
Diabetes mellitus is one of metabolic disorders characterized by hyperglycemia
resulting from defects in insulin secretion, insulin action, or both. The chronic
hyperglycemia of diabetesis associated with dysfunction, and failure of variousorgans.
Diabetic nephropathy (DN), is a serious microangiopathic complication of
diabetes mellitus, and it is leading to end stage renal disease. The development of DN
is highly influenced by genetic factors and hyperglycemia. Methylenetetrahydrofolate
reductase (MTHFR) is an enzyme important for Homocysteine metabolism. Severe
MTHFR deficiency is associated whith hyperhomocysteinemia. The MTHFR C677T
gene polymorphism causes an alanine to valine amino acid substitution, this results in
a thermo labile MTHFR enzyme with reduced catalytic activity.This mutation may be
associated with hyperhomocysteinemia. This mutation and hyperhomocysteinemia
were the concern of many studies to asses if they were associated with DN in type 2
diabetes mellitus patients.
This study was conducted on 50 persons, 20 control normal persons and 30
type 2 diabetes mellitus patients. The MTHFR C677T gene mutation was detected by
PCR-RFLP. The results showed that the presence of MTHFR C677T polymorphism
(CT+TT) is not significantly related to increased risk of development of
macroalbuminuria nor or microalbuminuria. There is no association of polymorphism
and macroalbuminuria. The presence of 677T allele also not significantly associated
to increased risk of developing macroalbuminuria.