الفهرس 
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Abstract
The current study focuses on The envelope glycoprotein D of Equine herpes virus-1 as it is considered one of the most potent inducers of virus-neutralizing antibody among the spectrum of EHV-1 proteins The current study utilizes the gene synthesis technology to synthesis the glycoprotein D of the Kentucky reference strain to use it as appositive control cloned in pMA-T plasmid for optimizing the detection of the virus in field samples by PCR using 2 sets of primers that flanks the coding sequence of about 1209 bp while t is 1000 bp , positive samples were sent for sequence analysis, and revealed nucleotide substitution at the base pair number 121 from the start codon which give lead to single Amino Acid Substitution from CAG (Glutamine(Gln/Q)) to AAG (Lysine(Lys/K)) then the sequenced product cloned Into PET 151 D topo plasmid with an N-terminal tag containing V5 epitope and 6xHis tag and transformed into Top 10 Ecoli and transformed again in Ecoli BL21 cell which include the T7 promotor for expressing the glycoprotein D, western blotting carried out on the induced culture at different expression times using the alkaline phosphatase labeled N-terminal anti histidine monoclonal antibodies and serum collected from aborted and infected mares revealed band at the expected site using anti equine IgG labeled horse radish peroxidase, concluding that the expressed envelope glycoprotein D of EHV-1 (EHV-1gD) could be used as a good candidate for manufacturing of diagnostic Antigen for detection of the circulating antibody either in vaccinated or latent infected horses.