الفهرس 
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Abstract
The current study was carried out to study the most important pathological changes in
liver and kidneys in animals infested by Fascioliasis and to detect Fasciola antigen in livers
and kidneys specimens. The total 80 cases of liver affected by Fascioliasis showed different
lesions in livers. The most important pathological changes in livers varied from acute to
chronic liver cirrhosis and the detected antigen by using immunoflurescent using (SigmaF7887) whole molecule IgG antibbovine antibody-FITC produced in rabbit revealed antigen
in liver capsules, liver cells, sections of mature and immature worm and in fibrotic portal
tracts and hepatic artery. The immunoperoxidase gave positive results in the same regions In
65 case of. Kidneys specimens of same animals revealed the most important associated
lesions which were proliferative glomerulonephritis, crescentic glomerulonephritis
mesangiaproliferative glomerulonephritis, membranoproliferative glomerulonephritis, the
renal blood vessels showed perivascular fibrosis, vaccuolation of the medial wall, thickening
of some medial renal blood vesssls and hypertrophied by smooth muscle cells proliferation.
The immunohistochemistry gave positive results in 50 cases of 65 case which exhibit lesions
in kidneys (glomerular lesions, interstitial lesion, renal tubular changes and changes of renal
blood vessels). The immunoflurescent using (Sigma-F7887) whole molecule IgG antibbovine
antibody-FITC produced in rabbit gave positive results in renal tubules epithelial layers and in
endothelial lining and adventitial layer of renal blood vessels. The immunoperoxidase results
using adult worm crude antigen gave +ve result in glomerular tuft, renal tubules epithelial
layers and in endothelial lining and adventitial layer renal blood vessels. The
immunoperoxidase results using excecretory-secretory antigen E/S gave positive results in
renal tubules and in endothelial lining and adventitial layer of renal blood vessels.