الفهرس 
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Abstract
The present study was conducted to investigate presence of C. burnetii in ruminant farms in Egypt. It was carried out on 319 apparently healthy ruminants’ animals (cattle, sheep, goat and camels) and 219 diseased ruminants (aborted and mastitic) for detection the C. burnetii. The samples were collected from 9 governorates in Egypt. A total of 315serum samples (205 from apparently healthy ruminants animals and 110 from aborted ruminants) 104 Bulk Tank Milk samples (56 from apparently healthy ruminants and 48 from aborted and mastitic ones),75 vaginal swabs (22 from apparently healthy cattle and 53 from aborted ruminants),36 fecal samples from apparently healthy cattle and 5 placenta from aborted ruminants . the milk and serum samples were examined serologically for determination C. burnetii antibodies using ELISA. The results of the serum samples of apparently healthy ruminants were 37 (12.9%) as (10% for cattle, 34.1% for sheep, 17.7% for goat and 10% camel), while the results of the serum samples of aborted and mastitic ones were 21 (19 %) as follow (20% for each ewes and she camel , 16.7% for cows).The results of BTM samples of apparently healthy ruminants were 20 (35.7%) , while the results of the BTM samples of mastitic and aborted ones were 33.3% and 11.1%, respectively.C. burnetii antigen was detected in 8 (14.3%) and 1 (4.5%) of 56 and 22 milk and vaginal swabs, respectively of apparently healthy cattle using RT-PCR. Also was detected in 3(10%), 1 (5.6%) of 30, 18 BTM samples from mastitic and aborted she camels, respectively. As well as it detected in 2 (11.8%), 2(11.5%) and 1(10%) from vaginal swabs of aborted ewes, she camel and cows, respectively using RT-PCR. C.b. Ag also detected in 1 (20%) of 5 placenta samples of aborted cows and 1(12.5%) of 8 internal organs of aborted ewes foeti. on other hand, C. burnetii antigen not detected in fecal samples from apparently healthy sheep and goat and not detected also in placenta of aborted she camels as well as internal organs of aborted she camels, cow foeti using RT-PCR.A total of 32 random samples from pervious positive and negative RT- PCR were propagated through inoculation of 7-day old ECE via yolk sac route inoculation. The inoculated samples confirmed by using RT-PCR and PCR, out of 32, 13 and 6 samples were positive to RT-PCR and PCR.