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العنوان
Prevalence of salmonella serovars in milk and table eggs /
المؤلف
Emara, Heba Mohamed El-Sayed.
هيئة الاعداد
باحث / هبه محمد السيد مصطفى عماره
مشرف / عادل عبدالخالق سيد أحمد
مشرف / أحمد محمد عبدالجواد الجمل
مناقش / مجدى شرف السيد
الموضوع
Salmonella. Milk. Salmonella food poisoning. Animal health.
تاريخ النشر
2016.
عدد الصفحات
88 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
01/01/2016
مكان الإجازة
جامعة المنصورة - كلية الطب البيطرى - Department of Food Hygiene and Control
الفهرس
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Abstract

This study aimed to investigate the prevalence of Salmonella and Antimicrobial Resistance profile in farm bulk milk and table eggs. Enterobacteriaceae are a large group of gram-negative rods bacteria that have a public hazard, as some of their members are pathogenic and cause public health hazard and food poisoning to human being. Furthermore, the Enterobacteriaceae count used as indicator of enteric pathogen such as salmonella and fecal E coli. This study included One hundred and fifty samples( 100 farm bulk tank milk samples and 50 table hen egg samples were obtained from dairy farms and different localities of Mansoura from the period between 2014 to 2015 .The collected samples kept in an insulated ice box (4±10C) to be transferred to laboratory for bacteriological examination The present work was carried out to study the prevalence of Salmonella spp. in 100 farm bulk milk samples collected from Mansoura city, Egypt. Salmonella was detected in 15% (15/100) of examined milk samples. Of the presumptive Salmonella isolates, 52 isolates were identified biochemically as Salmonella spp. Salmonella isolates were serotyped into Salmonella Typhimurium (12/52; 23.1%), Salmonella kentucky (11/52; 21.2%),Salmonella Enteritidis (8/52; 15.4%), Salmonella Heidleberg (6/52; 11.5%.), and Salmonella Infantis (6/52; 11.5%), while 17.3% (9/52) of isolates could not be typed. The sensitivity of the 52 Salmonella isolates against 11 antimicrobials revealed that 98% , 96% and 96% of isolates were sensitive to gentamycin, cephalexin and sulphamethoxazole/trimethoprim, respectively, while 92%, 33%, 29%, 25%, 24%, 20%, 17% and 16%, respectively were resistant to nalidixic acid, streptomycin, kanamycin, tetracycline, enrofloxacin, norofloxacin, amoxicillin and ampicillin, successively. The majority (50%) of Salmonella isolates were multi-drug resistant .In examined table eggs, the observed prevalence of Salmonella spp. in our study (18%) and out of 92 presumptive Salmonella isolates were identified biochemically as Salmonella spp. and confirmed serologically. Most of 92 Salmonella isolates were serotyped into 4 different serovars and the rest was untyped). Salmonella Newport was the most frequently isolated serotype with a ratio of 34.8% (32/92), followed by S. Typhimurium (32.6%; 30/92), while S. Enteritidis and S. Heidleberg were detected at a percentage of 17.4% (16/92) and 6.5% (6/92), respectively. Eight isolates (8.7%; 8/92), however could not be typed into serovars level. The results of disc diffusion test conducted for antibiotic resistance showed that nearly most of Salmonella isolates were sensitive to gentamycin (98% of isolates), cephalexin (97% of isolates) and sulphamethoxazole/trimethoprim (94%). Resistance to nalidixic acid, streptomycin, kanamycin, tetracycline, enrofloxacin and norofloxacin were 92%, 35%, 32%, 27%, 23% and 21%, respectively. Only small percentage of the isolates demonstrated resistance to amoxicillin (18%) and ampicillin (15%). The high resistance rates of Salmonella isolates against nalidixic acid, streptomycin, kanamycin, tetracycline, enrofloxacin and norofloxacin.