الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Marine fish plays an important role in the human diet with an observed increase in the consumption of marine fish. Thus, it is necessary to study the prevalence of pathogens in fish to ensure the safety of fish and fish products This study was carried out to evaluate the extent of contamination of some marine fish species via attempting of isolation and identification of some enteric pathogens therefore a total of 200 samples of marine fish including; Sardine (Sardina pilchardus), Barbone (Mullus surmuletus), Morgan (Pagrus pagrus) and Denise (Sparus aurata) (50 of each) were randomly collected from different markets in Alexandria Province. Each sample was kept in a separate sterile plastic bag and transferred directly without delay in an insulating refrigerated container (ice box) to the laboratory under complete aseptic conditions to avoid any changes in the quality of the samples. All collected samples were subjected to chemical and bacteriological examination The obtained results revealed that: 1. E. coli was isolated from 16(32%), 19(38%), 15 (30%) and 13 (26%) of the examined samples of Sardine, Barbone, Morgan and Denise. There were 5 serotypes of Enteropathogenic E. coli isolated from the examined samples O86: K61 (B7), O111: K58 (B9), O124: K72 (B17), O26: K60 (B6) and O128: K67 (B12) 2. Salmonella was isolated from 2(4%), 6 (12%) and 3(6%) of the examined samples of Sardine, Barbone and Morgan and there was no isolation in Denise. There were 4 serotypes of Salmonella isolated from examined sample of marine fish, S. Enteritidis, S. Typhimurium, S. Paratyphi and S. Haifa 3. Shigella species was isolated from 8(16%), 11(22%), 6 (12%) and 5(10%) of the examined samples of Sardine, Barbone, Morgan and Denise respectively. There were 4 strains of Shigella isolated from the examined samples of marine fish, Shigella flexneri, Shigella dysenteriae, Shigella boydii and Shigella sonnei. 4.Yersinia species were isolated from 26(52%), 34(68%), 21 (42%) and 16(32%) of the examined samples of Sardine, Barbone, Morgan and Denise, respectively. There were 4 strains of Yersinia isolated from examined samples of marine fish including Y. enterocolitica, Y. frederiksenii, Y. ruckeri and Y. intermedia. 5. Vibrio species were isolated from 12 (24%), 14(28%), 13(26%) and 8 (16%) of the examined samples of Sardine, Barbone, Morgan and Denise, respectively. There were 2 strains of Vibrio species isolated from the examined samples of marine fish, V. parahaemolyticus was found in 42 samples 10(20%), 13(26%), 11(22%), 8(16%) of the examined samples of Sardine, Barbone, Morgan and Denise, respectively. While V. cholera strain was found in 5 samples including 2(4%), 1(2%) and 2(4%) in Sardine, Barbone and Morgan but Denise was free. 6. Aeromonas hydrophilia was isolated from 6(12%), 8(16%), 9 (18%) and 5(10%) of the examined samples of Sardine, Barbone, Morgan and Denise, respectively. On the basis of the previously mentioned results, enteric pathogens still constitutes common contaminants of marine fish and its presence is very important due to its public health significance, good hygienic measures must be applied to obtain safe and wholesome meat.