الفهرس 
List of abbreviations
Organic wastesOnly 14 pages are availabe for public view
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Abstract
The aim of the present study was to obtain active fungal isolates having both cellulolytic activity to degrade rice straw (RS) and phosphate solubilization of rock phosphate (RP) this could be achived by isolation of ninety nine fungal isolates from three samples represent three-storage period of RS in this work. Aspergillus species represented the highest ratio among all fungal isolates with frequency equal to 30% followed by Fusarium, Penicillium and other species with frequency equal to 20%, 10% and 50%, respectively. All fungal isolates were screened for cellulases activities on agar plate by plate screening assay and measuring of the diameter of growth zone and diameter of hydrolysis zone and which can help and facilitate in calculation of the enzymatic index (EI) for each fungal isolate. from this only seventy fungal isolates that could be grown on CMC agar plate and both growth zone and clear zone could be distinguished easily by using Congo red dye. As. niger MY7 No. 81 and Stemphylum sp. MY31 No. 33 were the most species that have the highest value of EI equals to 2.01 and 1.93, respectively. from this could observed that Botrytis sp. MY29, P. purpurogenum MY48, As. niger MY55 and As. niger MY81 were the most fungi have both cellulolytic activities and phosphate solubilization then setting the cellulase “CMCase” and RP solubilization under SSF conditions to make time course profile. Identify two higher strains, which have good efficiency in production of some organic acids after that demonstration of the optimum conditions for the highest production of some organic acids and setting a good design through which to make optimization for high degradation of RS to maximize of some organic acids production. Applied to P. purpurgnium MY48 and As. niger MY81 in (SSF) under response surface method (RSM) by using Box- Behnken design with three independent variables purpurogenum MY48 and As. niger MY81 by using HPLC technique. Analysis illustrated to present of two organic acid were malic and succinic acids in case of P. purpurogenum MY48 with amount of 0.453 and 0.363 (mg / g RS), respectively. After this in case of As. niger MY81 analysis by HPLC illustrated the presence of four types of organic acids i.e. glycolic, malic, malonic and formic acid with amounts of 0.786, 0.341, 0.288 and 0.306 (mg / g RS), respectively. The final step concluded making of microscopical examination by using scanning electron microscope “SEM” for P. purpurogenum MY48 and As. niger MY81 to try and find out the morphological characters of them.