الفهرس 
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Abstract
This work aimed to the use of the resistant fungi and bacteria in biosorption experiments for removal of heavy metals which cause great harmful to both environment and human health, so it was important to find a safe and cheap technique to remove these elements from the environment. The chromium and lead resistant fungi and bacteria were isolated from the wastewater and sediment samples of the four investigated factories namely Delta fertilizer factory (DFF), Talkha electric power plant (TEP), Abnaa Zekry Marble and Granite factory (MGF) and Sandoup oil and soup factory (SOSF) in El-Dakahlyia Governorate. Ten fungal species in addition to 102 bacterial isolates were recorded from the four investigated factories seasonally. Seventeen different isolated microbes were chosen in addition to bakery yeast to perform screening biosorption experiment. A dummy variable (empty alginate beads) was used to evaluate the standard error of the design and as a blank to confirm that the biosorption capability. According to the results obtained, the most potential microbes to be efficient in removing chromium ions were found to be closely related to bacterial genera Bacillus, Vibrio, Paenabacillus and Serratia in addition to the fungal species Trichoderma viride, while the most potential microbes to be efficient in removing lead ions were found to be closely related to bacterial genera Vibrio, bakery yeast and fungal species Fusarium oxysporum. The used microbial consortium achieved a 100% removal of Cr (IV) ions from the industrial effluents within 10 minutes (approximately 60% after 5 minutes), while only 55% of Pb ions were removed in the proposed process. Applying the same condition on the TEP effluent samples in summer season showed a compelet removal of chromium ion and removed half of the lead. Different flow rates and initial pH were examined to determine the optimum condition for heavy metal removal. Using the generated mathematical model and graphs, the optimum removal conditions were found to be pH = 6-7 and Flow rate =1-5 ml/min.