الفهرس | Only 14 pages are availabe for public view |
Abstract Hend Abou Elazm ELsayed Ibrahim. Gene Expression of some Economic Traits Related to Heat Stress in Camel, Unpublished Ph.D. Thesis, Department of Genetics Faculty of Agriculture, Ain Shams University, 2016. Camels have a number of adaptations to help them to survive the most successfully in such dry and arid climates. Camels have an exceptional ability to adapt to desert lifestyle with remarkable physiological characteristics such as fluctuating body temperature from 34°C to 41.7°C throughout the day, tolerating water loss greater than 30%, and capability of drinking 100 liters of water in as little as 10 minutes. In the present study, global methylation was assessed in camels exposed to two climatic changes; cold and heat. Data obtained indicated the differences in the methylation of these animals, which, in turn, reflects a difference in gene expression patterns based on epigenetics. In winter season, the majority of samples enrolled in this study were hypermethylated while in summer season, half of the samples were hypomethylated and the rest were hypermethylated where there was a significant difference between samples in winter and summer with pvalue < 0.05 (p = 0.020). Meanwhile, a significant difference between samples in winter and summer on the level of gene expression of (leptin and IGF) with p-value < 0.05 (p = 0.000) has been obtained. The seasonal variation affected the levels of plasma proteins such as Immunoglobulin (γ-globulin), Post-Transferrin, Transferrin (β-globulin) and α-globulin (slow and fast). This study was concerned mainly with to genes (lipten and IGF), which are related to milk production, as an example of economic traits. The results obtained indicated that the reduced temperature (winter) has caused the whole genome of camels to hypomethylate while the elevated temperature caused hypermethylation of the whole genome. Real time- RCR data confirmed the epigenetics data with regards the two genes under study. We concluded that heat stress could regulate the gene expression on the epigenetics level |