الفهرس | Only 14 pages are availabe for public view |
Abstract It has been showri that granulomatous hypersensitivity reaction in tuberculosis (T.B) is mainly associated with THl cytoi<ine expression while that in schistosomiasis is TH2 depende1it. On the otl1er hand most of the adhesion molecules which regulate leukocyte I activati011 and migration are inducible by these cytoki.nes. This work investigates the granulon1atous inllammation and compares the expression of intercellular adhesion molecule·! 1 (ICAivl-1) and phtclct cndolhclial cell adhesion molecule-! ~ (PECAM-l) between the TB and schi~tosome models. /\n in vivo I granuloma formation was induced in immunized C)) 1 mice by I intra~enous. injection of sepharose beids coated .wilh either purified protem denvaltves (l’J>D) of ’¥· tuberculosis (m the TB moqel) or I . with soluble egg, antigens (SEA) of S. mansoni (in the schistosome model). Fro7.en lung sections were analy:.::ed for U1c c~p•ession of . \CAM-I and PECJ\M-1 using image analyzer. The two models I demonstrated significant increase in TCA M-1 expression in immunized animals that received antigen coated beads as compared to uncoated beud controls (p<O.OOl) and to naive controls I (p<O.OOl). In contrast, PECAJv(-l expres~ion was down1:cgulated in the schistosome model but not in the TB model. Tlu: net gnmuluma size formed aJ.·ow1d antigen coated beads in the vaccinated animals of I he two models wa~ significantly larger than that formed in the naive control animals or thll t fom1ed <~round ant ig::;n uncoated beads. lt is suggested lhal the diiTerence in PECAM-1 expression between the two · models might be due to different cytokine regulatory · mechanisms. |