الفهرس 
Review of literatureOnly 14 pages are availabe for public view
 |  | from | 32 |  |  |
| | | from | 32 | | |
Abstract
This work has been carried out in the Molecular Genetics and Genome Mapping Lab., and the experimental green house of the Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt during the pe1iod from 1993-1998, with the aim to:
1- Identify DNA sequence polymorphisms between the parents used in this study (L.esculentum x L.pennellii).
2- To detect molecular markers.
3- To try and develop STS around gene(s) of agronomic importance.
4- To develop a linkage map of tomato (L.escu!entum x
L.pennellii), using RAPD and STS markers.
5- An attempt was made to lise BSA for identification of markers associated with resistance to whitefly.
The principle results are summarized below:
Genetic variability between the parents:
The genetic variability between the parents was studied using RAPD analysis. Fifty decamer primers were used to screen the parents. All of the fifty RAPD primers successfully amplified bands in both parents, and all of them generated polymorphic bands between (L. escu!entum and L.penne!lii). The total number of reproducible fragments by the fifty primers reached 589, including
118 non-polymorphic fragments. This represents a level of polymorphism of80% and an average of9.42 markers per primer.
Identification of RAPD markers:
RAPD markers were identified between L.esculenf!m1 and
L.pennellii using 22 decamer primers. These 22 primers generated
105 R_APD markers with an average of4.8 markers per primer.
1- --•----------
89
Development of ,fo,’TS:
Ten pairs of primers, each one of them, about 25 base-pair long, were designed from the published tomato nucleotide database sequences. Each primer set was used to amplifY a sequence tagged site (STS) from the total DNA of L.esculenlum and L.pennellii. Informative primers were then used to screen for polymorphisms in the F, progeny of the same cross. Segregation data based on PCR amplification from STSs were analyzed for linkage with the data for RAPD markers.
Bulked Segregant Ana(vsis (BSA):
Lastly an attempt \vas made to use Bulked Segregant Analysis (BSA), for identification of markers associated with resistance to whitefly. Four primers gave positive results between the two bulks for resistance and susceptibility, three of which were mapped to their respective linkage groups and chromosomes.