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العنوان
Value of the Sperm chromatin Dispersion Test in Intra cytoplasmic Sperm Injection Outcome \
المؤلف
Abdel- Hay, Tamer El-Sayed Abdel-Hamed.
هيئة الاعداد
باحث / Tamer El-Sayed Abdel-Hamed Abdel- Hay
مشرف / Khalil Abd El-hamed El- halfaway
مشرف / Bahgat Abd Elghafar EL-fiky
مناقش / Yousry A. Dwidar
مناقش / Mohammed Fathey Fa
الموضوع
Spermatogenesis. Spermatogenesis- physiology.
تاريخ النشر
2015.
عدد الصفحات
72 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Molecular Biology
تاريخ الإجازة
1/9/2015
مكان الإجازة
جامعة مدينة السادات - معهد بحوث الهندسة الوراثية - Molecular Biology Department.
الفهرس
Only 14 pages are availabe for public view

from 32

from 32

Abstract

Sperm chromatin / DNA integrity is essential for the accurate transmission of paternal genetic information, and normal sperm chromatin structure is important for sperm fertilizing ability. The routine examination of semen, which includes sperm concentration, motility and morphology, does not identify defects in sperm chromatin structure. Evaluation of sperm DNA damage appears to be a useful tool for assessing male fertility potential both in vivo and in vitro.
So, the present study aimed to assess human sperm chromatin status in the ejaculated sperm of patients who suffer from infertility by using light microscope & sperm chromatin dispersion test and determination the relationship between sperm chromatin status and ICSI outcome.
Results obtained from the study summarized as follows:
1-The % of fertilization rate in abnormal sperm DNA in 17 patients suffering from infertility was (81.086±13.302) compared to normal sperm DNA in 19 patients suffering from infertility which was (73.857±24.289).
2-the division rate in abnormal sperm DNA in 17 patients suffering from infertility was (44.907±21.039) compared to division rate in normal sperm DNA in 19 patients suffering from infertility which was (51.496 ±24.496)
3- The % of pregnancy rate in abnormal sperm DNA in seventeen patients suffering from infertility showed that eight of seventeen responded to pregnancy (47.06% ) and nine not responded to pregnancy (52.94%) compared to normal sperm DNA in 19 patients suffering from infertility which showed pregnancy response of nine (47.37 % ) and non-response of ten (52.63%).
4-Abortion rate in abnormal sperm DNA in patients suffering from infertility showed that six cases of eight not complete pregnancy and aborted (75%) while two complete pregnancy and not aborted (25%) while normal sperm DNA in patients suffering from infertility showed that one case of nine not complete pregnancy and aborted (11.11%) and eight complete pregnancy and not aborted (88.89%).