الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 32 |  |  |
| | | from | 32 | | |
Abstract
A great challenge arises when sex determination of human remains is needed, especially when, only, bone fragments or isolated teeth are available; such as in mass disasters. The dental hard tissue physically encloses the pulp and offers an anatomical configuration of great durability that provides a potential source of genetic evidentiary material.
The aim of the present work was to extract and analyze the DNA from dental pulp for sex determination of a sample of Egyptian population using polymerase chain reaction (PCR)amplification of the sex determining genes; DYS14 and SRY.
Forty molar teeth (sound and carious) were included in the study. They were freshly extracted from adult Egyptians of both sexes, for various orthodontic, periodontal or prosthetic indications. The age of the patients ranged from19 to 58 years with a mean of 33.65 ± 10.61.Informed consent was obtained from patients for including their teeth in a DNA study for sex determination indicating that the samples will not be used for any purposes other than that of the study.
DNA was extracted from the pulp of the studied teeth using QIAamp® DNA Investigator Kit. DYS14 and SRY genes were PCR amplified for sex determination as they are Y chromosome-specific genes.
Androgen receptor (AR) gene was amplified as an internal positive control to confirm successful DNA extraction. A negative control was used throughout the experiments in the form of molecular biological water to detect any possible contamination.