الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Cryptosporidium is a genus of apicomplexan protozoans that can cause gastrointestinal illness with diarrhea in animals and human. The prevalence rate of Cryptosporidium species infection associated with some parameters such as sampling, age, seasons, country and contact to domestic animals. Cryptosporidium oocysts are 4-6 µm in diameter and exhibit partial acid fast stain. This study aimed to determine prevalence and genotype of Cryptosporidium species which infect human and animals” cattle, bovine and calves” in sharkiya Province, Egypt. Across sectional study was performed to detect the prevailing Cryptosporidium copro-DNA amplified and genotype using Restriction Fragment Length Polymorphism ( RFLP-PCR) .
This study aimed to determine prevalence of Cryptosporidium species which infect humans and animals” cattle, bovine and calves” in sharkia province, Egypt.Stool samples ( 142) are randomly collected from 27 cattles, 35 buffalos and 30 calves, as well as 50 human sample in different seasons from sharkia province, Egypt. Stool samples were examined by using PCR targeting and acid fast stain, ”coproscopically examined concerning the presence of Cryptosporidium species”. The overall estimated prevalence of Cryptosporidium in human was 28 % and 27% in animals. PCR identified Cryptosporidium hominis as genotype 1 and Cryptosporidium parvum as genotype 2.