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العنوان
Epidemiological Studies On Diarrhea In Cattle Farms =
المؤلف
Awad, Warda Khamees.
هيئة الاعداد
باحث / ورده خميس عوض
مشرف / حامد عبد التواب سماحه
مشرف / ياسر نصر عوض حجاج
مشرف / محمد السيد عبد اللطيف نصير
مناقش / سامي عبد العزيز الميداني
مناقش / موسي عبد الله محمد علي أيوب
الموضوع
Animal Hygiene.
عدد الصفحات
71 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
29/3/2016
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - صحة الحيوان
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study was carried out in 2 cattle farms; the first farm was located in Abbis city, Alexandria Province and the second farm was located in Motobis district, Kafr El-sheikh Province during the period extended from May to October, 2015. A total of 100 fecal samples were collected from diarrheic (70) and apparently healthy (30). Full history for each case was taken from owners including age, sex and health status. Fecal samples should be fresh, preferably collected from the animal during the act of defecation or from the rectum using a fecal loop during the physical examination. Samples were transported under possible aseptic condition with the minimum of delay to the laboratory of the Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Alexandria University where they were subjected to bacteriological and parasitological examination. The obtained results in the current study revealed that: • The frequency distribution of pathogenic bacteria isolated from fecal samples of cattle (n=100) revealed the isolation of E. coli, Salmonella spp., Shigella spp., Proteus spp. and Klebsiella spp. at the percentage of 31, 7, 7, 16 and 11 %, respectively while, mixed infection was noticed in 23% of the examined fecal samples. • The frequency distribution of pathogenic bacteria isolated from fecal samples of cattle in relation to health status revealed that the rate of E. coli and Salmonella spp. from diarrheic cattle (32.86% and 11.43%, respectively) was higher than apparently healthy cattle (26.67% and 6.67%, respectively). On contrary, the rate of isolation of Proteus spp. and Klebsiella spp. from diarrheic cattle (12.86 and 7.14%, respectively) was lower than apparently healthy cattle (23.33 and 20%, respectively). In addition, Shigella spp. was isolated from diarrheic cattle only at the rate of 10%. • The frequency distribution of pathogenic bacteria isolated from fecal samples of cattle in relation to sex revealed that: o The rate of isolation of E. coli was higher in females (41.67%) than in males (15%).o The rate of isolation of Salmonella spp. was higher in females (10%) than in males (6.67%). The rate of isolation of Shigella spp. was higher in males (6.67%) than in females (5%). The rate of isolation of Proteus spp. was higher in females (15%) than in males (11.67%). The rate of isolation of Klebsiella spp. was higher in males (15%) than in females (8.33%). The frequency distribution of pathogenic bacteria isolated from fecal samples of cattle in relation to age groups showed that: The rate of isolation of E. coli was higher in the age group (˂ 1 year) (33.33%) followed by the age group (˃ 2 years) (28.57%) and finally the age group (1-2 years) (25%). The rate of isolation of Salmonella spp. was higher in the age group (˃ 2 years) (14.29 %) followed by the age group (˂ 1 year) (9.09%) and finally the age group (1-2 years) (8%). The rate of isolation of Shigella spp. was higher in the age group (˃ 2 years) (14.29 %) followed by the age group (˂ 1 year) (7.58%) but it could not be isolated from fecal samples of the age group (1-2 years) (8%). The rate of isolation of Proteus spp. was higher in the age group (˃ 2 years) (35.71%) followed by the age group (1-2 years) (25 %) and finally the age group (˂ 1 year) (9.09%). The rate of isolation of Klebsiella spp. was higher in the age group (˃ 2 years) (14.29%) followed by the age group (1-2 years) (12%) and finally the age group (˃ 1 year) (9.09%). Serological identification of ten isolates of E. coli revealed the presence of O25 K11 (2), O111:K58, O101:K99, O78:K80, O125:K70 and 4 non-serotyped isolates Serological identification of seven isolates of Salmonella revealed the presence of S. Typhimurium (2 isolates) and S. Anatum (5 isolates) • Antimicrobial sensitivity testing of E. coli isolates revealed that tested isolates were highly sensitive to Gentamycin, Cefatoxime and Marbocyl, moderately sensitive to Oxytetracycline and Spectinomycine while less sensitive to Amoxycilline + clavulanic acid and Norfloxacin while for Salmonella isolates it was found that the tested isolates were highly sensitive to Enroflox and Marbocyl and moderately sensitive to Amoxycilline + clavulanic acid while less sensitive to Gentamycin, Cefatoxime, Tetracycline and Spectinomycine. The frequency distribution of parasites detected in fecal samples of cattle by direct microscopic examination revealed the presence of Eimeria spp., Ascaris spp., Strongyloides spp. and Fasciola spp.at the rates of 20, 11, 8 and 7%, respectively The frequency distribution of parasites detected in fecal samples of cattle by direct microscopic examination in relation to health status revealed that the recovered parasites were detected in a higher rate in diarrheic cattle than apparently healthy cattle. The frequency distribution of parasites detected in fecal samples of cattle by direct microscopic examination in relation to sex revealed that the rate of detection of Eimeria spp. and Fasciola spp. was higher in females than in males while the rate of detection of Ascaris spp. and Strongyloides spp. was higher in males than in females The frequency distribution of parasites detected in fecal samples of cattle by direct microscopic examination in relation to age groups revealed that: The rate of detection of Eimeria spp. was higher in the age group (1-2 years) (35.5%) followed by the age group (˃ 2 years) (28.57%) and finally the age group (˂ 1 year) (13.64%). The rate of detection of Ascaris spp. was higher in the age group (˃ 2 years) (28.57%) followed by the age group (1-2 years) (20%) and finally the age group (˂ 1 year) (4.55%). The rate of detection of Strongyloides spp. was higher in the age group (1-2 years) (25%) followed by the age group (˃ 2 years) (14.29%) and finally the age group (˂ 1 year) (1.52%) The rate of detection of Fasciola spp. was higher in the age group (˃ 2 years) (21.43%) followed by the age group (1-2 years) (15%) and finally the age group (˂ 1 year) (1.52%).