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العنوان
Studies on polysaccharides from Pleurotus salmoneo-stramineus /
المؤلف
Enas Mohamed Abd EL-Moneam EL-Gaar
هيئة الاعداد
باحث / إيناس محمد عبد المنعم عطية الجعار
مشرف / سعاد هحود أبو السعود
مشرف / إيواى حسي فخحى عبد الظاهر
مشرف / عزة عبد الرحوي هصطفى
الموضوع
Botany.
تاريخ النشر
2016.
عدد الصفحات
p 205. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة طنطا - كلية العلوم * - Botany
الفهرس
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Abstract

Exopolysaccharides and intracellular polysaccharides were extracted from Pleurotus salmoneo-stramineus. By testing three types of media for high production of EPS and IPS, it was found that glucose yeast peptone medium was the preferred medium for production under shaked and static condition. 150 rpm approved high production of EPS and IPS. Maximum production of EPS and IPS under different testing incubation periods were at 10 days. By studying different initial pH under shaked condition, it was found that EPS and IPS were optimum at pH 6 and 5.5 respectively. One and two disc (1cm) of the tested organism were suitable for high production of EPS and IPS dry weight. By testing different volumes of GYP medium on the maximum production of EPS and IPS, 50 and 100 ml were the best respectively. At 25 and 30°C, maximum EPS and IPS dry weight were achieved. Glucose, fructose, sucrose, maltose and starch were tested for EPS and IPS maximum production, EPS and IPS dry weight were high by cultivation organism on media containing sucrose and maltose as carbon source. At different concentrations of sucrose and maltose, 78, 90 mg/ml were the best for high production. Different nitrogen source including organic and inorganic source such as urea, sodium nitrate, yeast extract peptone powder, glutamic acid and aspargine were checked for its efficiency in maximum EPS and IPS dry weight, Yeast extract peptone powder was the optimum nitrogen source for them. Different Yeast extract peptone concentrations were tested, 33.8 and 54 mg/ml were the suitable concentration for both EPS and IPS respectively.