الفهرس 
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Abstract
strains were designated as UFOlO, UFO!!, UF012, UF013 and
Frankia UFOOl, UF002 were used as a reference strains. By light microscope all the isolates
exhibited similar Frankia structure hyphae, sporangia and vesicles, being able to fixing
atmospheric nitrogen. The growth patterns of the isolates and strains in BAP medium were similar to
Frankia growth pattern. Differentiation between the four Frankia isolates and the two strains by
their electrophoretic patterns of total protein , intrinsic antibiotics resistance test using
(kanamycin sulfate, nalidixic acid, gentamycin sulfate, tetracycline, neomycin sulfate,
chlramphenicol, streptomycin sulfate), their ability to use other carbon sources such as (sodium
propionate, galactose, lactose, marmose, maltose, marmitol, sucrose, fructose, sodium citrate,
sodium pyruvate) and electrophoretic analysis of the total DNA and plasmid DNA were carried out.
Reliable nodulation, number of nodules, dry weight of nodules and ARA of C. cunninghamiana
seedlings were obtained by inoculating them with Frankia hyphae under defmed greenhouse conditions,
among the varrous morphological cell types (spores and vesicles).
Casuarina glauca and C. cunninghamiana inoculation with Frankia hypha and/or mycorrhizae were grown
in loamy sand and clay soils under greenhouse conditions. High nodulation, ARA and growth
parameter of plant were obtained with inoculation with Frankia and/or mycorrhizaefd.