الفهرس 
Chapter 1Only 14 pages are availabe for public view
 |  | from | 16 |  |  |
| | | from | 16 | | |
Abstract
Diagnosis of filarial infection is for the most carried out by microscopic examination of dissected mosquitoes or of freshly engorged blood meals. More recently, a highly specific PCR-based study used to screen wild mosquitoes for infection with the worm, have registered divergent mosquito infection rates when determined by dissection and PCR. Discrepancy in infection data may be attributable to the detection of circulating worm DNA fragments within the mosquito. The present study aimed to test whether circulating W. bancrojii DNA fragments are detectable in Cx. pipiens fed an infected blood meal and proved free of filarial worms by dissection. Also, the Sspi-PCR assay was used, to evaluate W. bancrojii infection in Cx. pipiens collected from two villages in Qaliubiya Governorate with high and low prevalence of human
filariasis (EI-Qolzom 10.8% and Kafr Shorafa2.1 %)