الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
This study was planned to study the neurotoxic effect of AlCl3 on the brain of rats and the protective effect of Omega3 and Quercetin as antioxidant and the Aricept as drug for Alzheimer disease .Fifty male adult rats weighing (90-100g) were selected for this experiment. 1st group included rats that fed Purina rat chow ad libitum, which contains 3.3 kcal/g with 23.4% protein, 4.5 % fat, and 72.1 % carbohydrate in the form of complex polysaccharide for 2 months and served as normal control.2 nd group orally provided with AlCl3 (100mg/kg bw) for 2 months.3rd group, orally provided with AlCl3 (100 mg/kg bw) and given Quercetin (100 mg/kg of body weight) 2 months.4th group orally provided with AlCl3(100 mg/kg of body weight) and given omega 3 (20 mg/kg bw).Each capsule contained 180 mg (EPA) Eicosapentaenoic acid and 120 mg of (DHA) Docosahexaenoic acid that is total of 300 mg of Omega-3 fatty acid for 2 months.5th grouporally provided with AlCl3and the drug (Aricept) (2mg/kg bw) for 2 months. The animals were weighed on a weekly basis and the doses of chemicals and drugs were adjusted according to body weight and food intake. At the end of the experimental periods first month and the second month, brains were rapidly dissected, thoroughly washed with isotonic saline, and dried. Each brain was mid saggitally divided into two portions. The first portion was fixed in formalin buffer for histopathological investigation. While the second portion was weighed and homogenized immediately to give 10% (w/v) homogenate in ice-cold medium containing 50mM Tris–HCl and 300mM sucrose. The homogenate was centrifuged under cooling at 3000rpm for 10 min. The supernatant (10%) was used for determination of AChE, MDA and Reduced GSH. Section of the brain was dissected and fixed in 10% neutral buffered formalin, dehydrated in serial dilutions of alcohol and embedded in paraffin. Paraffin blocks were sectioned in duplicate at 5 μm and routinely stained by haematoxylin and eosin.There is a significant increase in Ach esterase activity and MDA levels with a significant decrease in levels of reduced GSH in brain tissues in the AlCl3 supplemented group when compared with control group. Administration of Quercetin, omega 3 or Aricept ameliorates the toxic effects induced by AlCl3 administration.
The result summarized here indicates that chronic ingestion of aluminium chloride leads to oxidative stress which is a hallmark of oxidative imbalance in the brain of individuals with Alzheimer’s. Toxic effects of AlCl3 such as neurotoxicity as a result of oxygen free radical generation, hippocampus apoptosis, can be alleviated by administration of Quercetin, omega 3 and Aricept.