الفهرس 
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Abstract
This study was done to evaluate the transmission and replication of live attenuated avian infectious laryngotracheitis vaccinal viruses derived from chicken embryo origin (CEO) and cell culture origin (CCO) into exposed contact chickens. Compare lesions induced post vaccination with that of experimental viral infection. Trial of detection and isolation of AILTV from vaccinated as well as contact chickens. Detection and identification of AILTV transmission and replication into contact as well as experimentally infected chickens using convential PCR. So in this aspect the work was conducted on using 84 one-day old Sasso chick obtained from private hatchery at Sharkia Governorate and reared under hygienic conditions till reached 44- days old then they were divided into 4 groups. The first group contained 24 chickens, 12 chickens were inoculated intra ocular with 0.1 ml of ILTV isolates and other 12 chicken kept as contact control group. The second group also contained 24 chickens, 12 chickens were vaccinated via eye DROP in one eye by CEO vaccine, and other 12 chickens kept as contact to them. The third group contained 24 chickens, 12 chickens vaccinated with CCO vaccine and other 12 chickens kept as contact to them. Four group contained 12 chickens (none infected and none vaccinated chickens) and kept as control negative during the previous work. Chickens in all groups kept under observation for 3 weeks PI and PV and sacrificed (3 chickens of each sub-group per day on each sacrifiy) on 3, 7, 15, 21 day PI and PV. All clinical signs and postmortem lesions were recorded. Specimens from conjunctiva, larynx, trachea and lungs were collected and fixed in neutral buffered formalin 10% and prepared for the routine paraffin histopathological technique. Tissue specimens (larynx, trachea and lung) were taken from both vaccinated and contact chickens of groups (2 and 3) on 7th day post vaccination and stored at -70ºC until processed for virological analysis.