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العنوان
GENETIC AND MOLECULAR STUDIES FOR charACTERS RELATED TO COTTON
LEAF WORM RESISTANCE
IN SOYBEAN \
المؤلف
ABDEL WAHAB,EMAN IBRAHIM EL SAYED .
هيئة الاعداد
مشرف / إيمان محمود فهمى
مشرف / رانيا أحمد يونس
مشرف / محمد سيد على محمد
مناقش / حسن محمد زكى علام
تاريخ النشر
2015.
عدد الصفحات
126p.;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة عين شمس - كلية الزراعة - قسم الوراثة
الفهرس
Only 14 pages are availabe for public view

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Abstract

A three year–field experiment was carried out in Giza Agric.
Exp. & Res. St., A.R.C., Giza Governorate, Egypt, during 2008, 2009 and 2010 seasons. Five soybean
genotypes (four resistant and one susceptible) and their hybrids were evaluated for the resistance
of cotton leaf worm (Spodopetra littoralis). Under field conditions, soybean plants were not
subjected to chemical control to determine percentage of rating levels of leaflets area consumed by
leaf feeding larvae of cotton leaf worm. This percentage also was determined under laboratory
conditions. The results indicated that soybean genotypes; Giza 111, Giza 22, L 158, L127, hybrid 4,
hybrid 5, hybrid 7 and hybrid 10 were resistant for cotton leaf worm, whereas, the susceptible
parent Dr–101, hybrids 1 and 6 showed the reverse trend. This study confirmed the relationship
between pubescence density and feeding preference by cotton leaf worm for leaflets of soybean
genotypes. High concentrations of Jasmonic acid in leaflets of the resistant soybean genotypes
reflected positively on the yield. SDS- protein PAGE exhibited bands 13 and 14 with MW of 55 and 40
KDa, respectively, as markers for the resistant parents and hybrids. The ISSRs analysis was
performed using 15 primers with soybean genotypes, yielded 135 and 91 total number of amplified and
polymorphic fragments, respectively. Five markers were detected using four primers for cotton leaf
worm resistance. The present study focused on the characterization of genetic diversity among the
tested soybean genotypes using SDS-protein and ISSRs-DNA markers to determine their resistance
and/or susceptibility to cotton leaf worm.