الفهرس 
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Abstract
Sheep pox virus is a highly contagious viral disease; one of the most important poxviruses listed in group A diseases of OIE. In Egypt, SPV one of disease that affects on livestock industry and although there is a vaccination program adopted against sheep pox still some foci of disease appeared.
In this study, diagnosis of SPV was based on clinical signs followed by virus isolation on ECEs, Vero cell cultures with further confirmation by EM-PCR and serological tests (SNT and ELISA).
Priminary diagnosis of poxvirus was detected by observed clinical signs on suspected diseased sheep. 46 (12.1%) sheep among 380 sheep from different localities at Sharkia province suffered from lesion resembling pox disease. On the other hand, there were no characteristic clinical signs among 116 goats leading to suspicion in poxvirus infection.
Regarding to isolation of the poxvirus on CAM of ECEs, 12 representive processed tissue homogenate obtained from infected sheep, revealed that 10 (83.3%) positive samples. The pathological changes on CAM were detected after 5 days from virus inoculation in the form of slight edematous thickening of CAM with apparent opaque white pock lesions that became clearer after 3rd passage. With comparison non-changes on negative control.