الفهرس 
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Abstract
This study was conducted on eleven healthy adult white New Zealand
rabbits with average weight 2-3kg. They were caged in experimental animal
house at Physiology Department, Faculty of Medicine, Alexandria University.
All rabbits were classified into two groups:
I- Control group: one unoperated rabbit served as controls
II- Study group: Included ten rabbits; two rabbits will be sacrificed at one
week, two weeks, one month, two months and three months.
All the animals were examined by a veterinarian to exclude any
diseased animal; they were weighted to estimate the amount of anesthetic
solution and all of them fasted the night before the surgical operation to
prevent vomiting after anesthesia.
The surgical procedure was done under complete aseptic conditions for
all rabbits. Autologous blood was aspirated from pinna vein and reinjected
into the superior joint space and peri capsular area, followed by external
ligature elastic for fixation in the first 24h.Finally, intra mascular injection of
antibiotic and analgesic to alleviate post operative pain and infection.
Each rabbit is sacrificed according to the post injection periods and the
obtained specimen are harvested and prepared for histological evaluation.
After one and two weeks, HE revealed slight decrease in cell number
and thickness of condylar cartilage, in addition to increase in fibrous layer as
compared with the control. No apparent increase in fibrous tissue collagen
fibers as encountered with trichrome stain.After one month, HE and trichrome staining showed regional loss of
chondrocytes. The cartilage layer showed degeneration and necrosis of
chondrocytes, while some areas show areas of calcification.
After two and three months, the cartilage zone is intact showing
regenerative changes with new cartilage formation, the fibrous layer is the
same thickness as that of the control and the bone region is similar to the
control with evidence of areas of new bone formation. The collagen fibers and
the fibrous layer are intact, no fibrous bed formation, no fibrous adhesions
and no remnants at all.
All of the data gained in this study support the superiority of injection
of autologous blood for the initial management of most chronic recurrent
TMJ.
It can be concluded that autologous blood injection serves as an
alternative, minimally invasive non-surgical procedure for the treatment of
chronic recurrent dislocation of the temporomandibular joint.
There are numerous advantages regarding autologous blood injections
for the treatment of chronic recurrent TMJ dislocation. Because there is no
incisions or tissue dissection were carried out. Autologous blood injection
compared to the invasive procedures where the susceptibility of facial nerve
injuries; altered sensation, swelling, infection & pain may exist.
Moreover, the injection of autologous blood to the TMJs could be
performed under local anesthesia or under intravenous sedation and there is no
need to hospital admission or general anesthesia. Autologous blood injection is a
simple technique that can be performed on an outpatient basis.