الفهرس 
AbstractOnly 14 pages are availabe for public view
 |  | from | 32 |  |  |
| | | from | 32 | | |
Abstract
Mycobacterium avium subsp. paratuberculosis (MAP) is the cause of Johne’s disease (JD), a chronic granulomatous intestinal disease of cattle. The disease represents one of the important problems facing dairy industry and causing high economic losses. 100 fecal and 100 serum samples were collected from JD clinically suspected cases. Sixty out of the 100 fecal samples were positive using Ziehl Neelsen stain showing acid and alcohol fast red bacilli.. Bacteriological examination of the 100 fecal samples reveled the isolation of 34 MAP isolates. The recovered MAP isolates were tested by conventional and nested PCR. The nested PCR proved the identity of the 34 MAP isolates; however, the conventional PCR using different primers gave variable results. Using a commercial MAP specific ELISA kits, only 13 out of the 100 serum samples were positive. The recovered MAP isolates were cultured on modified Middlebrook 7H9 and modified Watson Reid liquid media for preparation of the MAP cultural filtrate (CF) proteins, which were then separated by SDS-PAGE. The MAP CF immunogenic determinants were identified using western- Blot technique. Different immunogenic bands from 9-72 kDa were identified.