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العنوان
Studies on E.coli Infection In Broiler Chicken With Special Reference To Genetic Constituent /
المؤلف
Salem, Heba Nazeh.
هيئة الاعداد
باحث / هبه نزيه سالم
مشرف / محمد محروس مجاهد
مشرف / محمد عباس السيسى
مشرف / محمد عباس السيسى
الموضوع
Broilers (Chickens).
تاريخ النشر
2015.
عدد الصفحات
103 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Department of Avian and Rabbit Medicine.
الفهرس
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Abstract

The aim of this work which is carried out at El-Gharbia governorate, in several localities is to differentiate between avian pathogenic E.coli and non pathogenic E.coli in broiler chickens. As following: 1-To evaluate the occurrence of pathogenic types of E.coli among the diseased cases in EL-Gharbia governorate.2-Investigate the genetic differences in isolated E.coli strains using RAPD analysis. 3-Asses the prevalence of antimicrobial-resistance among isolated E.coli.
The main results of this study were: 1-Bacteriological examination of 150 broiler chickens revealed the isolation of 100 isolates of E.coli .Isolation on macconkey agar then isolation on EMB agar then isolation on congo red :all reveal typical E.coli character.2-API 20E applied on 20 isolate of E.coli revealed all strains were E.coli. API 20E is accurate test for biochemical identification of E.coli. 3-Serotyping of 15 of E.coli isolates revealed the following : (2 isolates) of E.coli O158 (13.3%), (2 isolates) of E.coli O63 (13.3%), (1isolate) of E.coli O8 (6.6%), (1 isolate) of E.coli O78 (6.6%), (2 isolates) of E.coli O169 ( 13.3% ), (1 isolate) of E.coli O151 (6.6%), (3 isolate) of E.coli O146 (20%), (1 isolate) of E.coli O159 (6.6%), (1 isolate) of E.coli O142 (6.6%), (1 isolate) of E.coli untyped.