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العنوان
Use of molecular techniques to detect viruses in different water bodies /
المؤلف
Rashed, Mohammed Kamal Abd EL-Alem.
هيئة الاعداد
باحث / محمد كمال عبد العليم راشد
مشرف / يحيي عبدالمنعم عثمان اللازق
مشرف / وليد مرسى السنوسي محمود
مشرف / عادل أحمد علي المرسي.
مناقش / احمد بركات بركات
الموضوع
Plant viruses. Virus diseases of plants. Molecular virology. Plant Pathology - Laboratory Manuals. Plant Viruses - genetics - Laboratory Manuals.
تاريخ النشر
2016.
عدد الصفحات
Previous pages :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة المنصورة - كلية العلوم - النبات
الفهرس
Only 14 pages are availabe for public view

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Abstract

Drinking water is one of the main sources of microbial pathogens in developing regions, which affect human health, furthermore there are many diseases associated with water. Many experiments are done to approve that no any pathogens found in drinking water. These experiments show the bacterial and physicochemical properties to water but don’t show the presence of viruses in water. There are many viruses found in water which called enteric viruses group that cause gastroenteritis for children and adult people. One of this group is rotavirus (RNA virus) which can pass through the treatment steps and can resistant to chlorine. Another virus is adenovirus which causes many diseases like gastroenteritis, childhood respiratory and conjunctivitis. Furthermore, adenovirus is DNA viruses which can more resistance to chlorine and treatment processes in water treatment plant. The present investigation was conducted to study the detection of the enteric viruses (especially, rotavirus and adenovirus) in different treatment steps of water treatment plant and show the relation between the bacterial indicators and those two viruses.
To address this aim Collect the water samples from treatment steps of traditional water treatment plant (Shark El- Mansoura) and two compact units (Depo Awam (American design) and El-Dnabik (Egyptian design ).Furthermore, There are three examinations to every water sample according to the Egyptian standard drinking water quality guidelines (Physicochemical, Bacteriological and Molecular examination).
The results of this study could be summarized as follow: All Physicochemical analysis for water samples were within the permissible limits of the Egyptian standard drinking water quality guidelines 2007. The total coliform, fecal coliform and fecal Streptococcus were not detected in chlorinated water of three WTPs and all samples were within the permissible limits of the Egyptian standard drinking water quality guidelines. Rotavirus and adenovirus existed in 13.8% and 38.8% out of 72 of total inlet water (raw water) samples, respectively. While, they were found in 11.11% and 40.2% out of 72 of total outlet water (drinking water) samples collected from the designated three WTPs.
Conclusion: The incidence of adenovirus is higher than the incidence of rotavirus in all three treatment plants. Adenoviruses have no peak, while rotaviruses have a peak during autumn and winter. The traditional water treatment plant has more efficiency than the compact units for removal of viral genomes and infectious units Bacterial indicators (total coliform, fecal coliform and fecal streptococcus) couldn’t be used as viral indicator.