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Abstract The selective photodynamic activity of each of methylene blue (MB and toluidine blue O (TBO) have been studied on Enterococcus faecalisE.faecalis) and BHK-21 fibroblasts as a model of mammalian cells. The photobactericidal activity of both dyes was assessed as a function of preillumination incubation time, dye concentration and total energy delivered by laser irradiation. Under all experimental conditions, the bacteria manifested higher sensitivity toward TBO mediated photosensitization, compared to that mediated by MB. SDS-Page analysis, revealed evident alteration in the electrophoretic pattern of the outer membrane integrity as a consequence of the photosensitization process. Transmission electron microscopy of the photosensitized bacteria revealed massive destruction of the bacterial cell wall with dissolution of the inner content. Exposing BHK-21 fibroblasts to the same protocols, which induced maximum photobactericidal effect revealed that the mammalian cells were relatively less sensitive for thephotodynamic process. |