الفهرس 
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Abstract
The present work was conducted to study the effect of He-Ne laser irradiation on the human seminal fluid. The literature was reviewed including laser physics, laser tissue interaction, laser biostimulation and seminal fluid The laser used in this study was He-Ne laser at wave length 632.8 nm and out put power 10 mW. The laser irradiation applied by scanning method. 30 ejaculates of semen, collected from the donors, each ejaculate divided to 6 aliquots the first is control and the remaining aliquots are irradiated with He-Ne laser at 2, 4, 6, 8 and 10 J/cm². All specimens investigated for Motility, Viability, Glucose, Fructose, GOT and LDH.In the present study the motility of sperm was assessed by CASA (computer assessed semen analysis) after 1, 3 and 6 hours incubation. The viability of sperms was assessed by Eosin-Nigrosin stain. It was found that the motility of sperms irradiated with 2,4 and 6 J/cm² significantly increased compared to control group by 12.1 %, 21.9 % and 9.7 % respectively (P value < 0.0001). While motility of sperms irradiated by 8 and 10 J/cm² decreased compared to control group by 19.6 % and 31.7 % respectively (P value < 0.0001). It was found that the viability of sperms irradiated with 2,4 and 6 J/cm² significantly increased compared to control group by 17 %, 28.5 % and 8.5 % respectively (P value < 0.0001). The viability of sperms irradiated by 8 and 10 J/cm² decreased compared to control group by 35.7 % and 48.5 % respectively SummaryThe consumption of glucose increased by 5.8 %, 17.3 % and 5.8 % with fluences of 2, 4 and 6 J/cm² respectively as compared to control group (P value < 0.0001) and decreased by 12.2 % and 14.4 % with fluences 8 and 10 J/cm² compared to control group. (P value The rate of Fructose consumption was increased by 3.8 %, 14.1 % and 2.5 % with fluences of 2, 4 and 6 J/cm² respectively as compared to non irradiated samples (P value < 0.0001) and decreased by 6 % and 12.6 % with fluences of 8 and 10 J/cm² than control group. (P value < 0.0001>In this studies the assessment of GOT and LDH showed insignificant changes as compared to control group up to 6 hours incubation In the present study it was found that the application of He-Ne laser irradiation at fluences ranging from 2 – 6 J/Cm² produced significantly increases of sperm motility, increased consumption of glucose and fructose, which used in the motility of the sperms through 6 hours incubation compared to the control group The application of He-Ne laser irradiation at fluences above 6 J/cm² produces gradually destructive effects on the sperms.