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العنوان
Isolation And Identification Of Proteus Species from Retailed Milk With Special Reference To Multidrug Resistant Strains /
المؤلف
Abd El Fatah, Safaa Samir.
هيئة الاعداد
باحث / صفاء سمير عبد الفتاح
مشرف / نشوى عبد السلام عز الدين
مشرف / خالد فاروق محمد العامرى
مشرف / أحمد سمير محمد
الموضوع
Milk. Proteus (Bacteria). Antibiotics.
تاريخ النشر
2015.
عدد الصفحات
70 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
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Abstract

In the present study, 151 milk samples (raw and pasteurized) were collected to investigate the prevalence of Proteus. The isolation of Proteus from milk samples revealed 13 isolates (8.6%) positive samples . Antimicrobial sensitivity testing against different antibiotic agents revealed that all of isolates (100%) were sensitive to imipenem, while 92% of the isolates were sensitive to nalidixic acid and ceftazidime, 84.6% to ampicillin/sulbactam,70.9% to sulphamethoxazole/trimethoprime, 69.2% to cefotaxime.
On the other hand, 61% of isolates were resistant to ampicillin followed by 53.8% to cephalexin.
Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that isolates were 6 positive for blaTEM gene , were 3 positive for blaCTX and were 5 positive for blaSHV gene and we do comparison between chromosome and plasmid and found ESBLs genes (blaTEM, blaCTX and blaSHV) in both sites.