الفهرس 
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Abstract
This study aimed to : Dissertation Abstract (one page A4). The present investigation was conducted to study biodegradation of petroleum wastes (lubricating oil, spent car oil) by using biological organisms, determine the best concentration of biodegradable lubricant for reduction the growth and sporulation of Alternaria solani in-vitro and controlling early blight disease by using biodegradable lubricant under greenhouse conditions. The experiment was conducted during 2014 and 2015 at Plant Pathology Department, Seed and Tissue Pathology Lab (SEPA), Central Laboratory, Faculty of Agriculture, Mansoura University, Egypt. Two pathogenic isolates of Alternaria solani namely Al-Tawfiqiyah and Badr were obtained from Plant Pathology Research Institute, Agricultural Research Centre (ARC), Ministry of Agriculture and Land Reclamation, Egypt. This isolates were tested for their pathogenicity, under controlled greenhouse conditions, on tomato plants, cultivar : Carmen F1. The disease incidence caused by Al-Tawfiqiyah isolate was significantly higher than that of Badr isolate. Al-Tawfiqiyah and Badr isolates produced the highest yield of conidia respectively with V-8 medium. Six compounds, namely phloroglucinol, phenazine, benzoquinoline, anthracene, phenol, and m--Cresol were identified in the final biodegradable lubricant by the aid of HPLC technique. For both Al-Tawfiqiyah and Badr isolates, complete inhibition (100%) of the mycelial growth ,spore germination and germ tube length were obtained by biodegradable lubricant at 220 µg/ml concentration. Under greenhouse conditions biodegradable lubricant at 220 µg/ml have significantly decreased disease incidence and disease severity and increased significantly of some growth parameters and yield of tomato plants as compared with Ridomil fungicide.This study recommended that treating of tomato plants with biodegradable lubricant at 220 µg/ml concentration.