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Abstract The obtained results demonstrated that the environmental seasonal variation affected the physico-chemical characteristics of water samples of the River Nile in El- Gharbia governorate and consequentially affected on presence of different bacterial and other microbial communities. The presence of different bacterial genera in the water of the River Nile at El-Garbia governorate is due to direct contamination caused by human activities and indirect effect caused by ecological disturbances. People discharge their domestic and/or agricultural wastes as well as human body wastes into river.The water of the River Nile in El- Gharbia governorate is subjected to fecal pollution and continuous monitoring of microbial quality of water is recommended to control the spreading of pathogens which transmitted by contaminated water. Seasonal variation on of various physical parameters of the collected water samples during December 2012 to November 2013 revealed that the highest value of temperature was recorded in summer (29.27 °C) and the lowest one was recorded during winter (17.94 °C). The highest pH value was recorded at spring (8.49) and the lowest one was (7.67) detected in summer. The highest EC was recorded during winter (493.44 μmhos/ cm ) while the lowest one was (424.78 μmhos/ cm ) detected in summer. The highest TDS value (323.89mg/L) was recorded during winter while the lowest one was (254.44mg/L) detected in spring |