الفهرس 
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Abstract
The main task of this study to evaluate the molecular and biochemical effect of the novel nanocomoposites in vitro and in vivo (Spirulina / CTAB / Zinc oxide) associated with mammary carcinogenesis induced by MNU (50 mg. / kg) body weight in female rats were investigated. Through evaluation of the following molecular and biochemical tissue and serum parameters: Molecular analysis of Cell Cycle, Caspase-3, P53, BCL2, TGF-B1, Mammary Levels of L-Malondialdehyde (MDA), Reduced Glutathione (GSH) and Activities of antioxidant enzymes (Catalase (CAT), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx)) , Activities of serum Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Serum levels of Nitric oxide (NO), Furthermore, histopathological changes of the mammary tissues were also investigated.
Preparations of Spirulina, Cetyl trimethyl ammonium bromide, Zinc Oxide Nanocomposites:
Preparation of Nanocomposite for biochemical investigation of antitumor activity as anticancer was prepared through the following methods.
3- Formulation of nanocomposite (A) : Spirulina (A), Cetrammonium bromide (B) were mixed together as follows:
(A) = 4 g
(B) = 2 g
4- Formulation of drug (B) : Spirulina (A), Zinc oxide (B) were mixed together as follows:
(A) = 4 g
(B) = 2 g
3- Formulation of drug (C): Spirulina (A) + Cetrammonium bromide (B) + Zinc oxide (C) were mixed together as follows:
(A) = 4 g
(B) = 2 g
(C) = 2 g
4- The formula drug was mixed mechanically very well with the using vortex then both are grind to the nano sized particles using ball mill (nanosystem unit) at 200 rpm for 10 hours.