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العنوان
Some Physiological Studies of Oocytes Maturation in One- Humped She-Camel (Camelus dromedarius) /
المؤلف
Mahmoud, Mayada Abdallah Elsayed.
هيئة الاعداد
باحث / ميادة عبدالله السيد محمود
مشرف / نــاديـة أحـــمــد طـــه
مشرف / فرانسوا أمين رياض ساويرس
مشرف / عبدالمحسن محمد همام
الموضوع
FCS educational circular.
تاريخ النشر
2015.
عدد الصفحات
148 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - physiology
الفهرس
Only 14 pages are availabe for public view

from 188

from 188

Abstract

About 338 ovaries were collected from Nahia and El-Warraq slaughter houses during December 2013 till May 2014 (breeding season). Excellent COC were matured in vitro in TCM-199 or Ham’s F12 media contained 10% fetal calf serum (FCS) or 10% fetal dromedary camel serum (FDCS) supplemented with different antioxidants 100µM/ ml β-mercaptoethanol (βME), 100µg/ ml royal jelly (RJ), 100µg/ ml ascorbic acid (Vit C), 100µg/ ml selenium (Se) and 100µM/ ml melatonin (MEL) under controlled condition (38.5 °C in 5 % Co2 for 24 hours in humidified air) . Recovery rate was 8.1 oocytes/ovary and the maturation rate was significantly higher in βME and Se supplemented TCM-199 medium enriched with FDCS compared to control medium (p<0.05), while there were no any significant difference found between the control group and the different antioxidant supplemented groups in TCM-199 medium enriched with FCS or Ham’s F12 medium enriched with FCS or Ham’s F12 medium enriched with FDCS. Additionally, TCM-199 has superior effect on the maturation rate of dromedary she camel oocytes than Ham’s F-12 and The FDCS has higher effect on the maturation rate of dromedary she camel oocytes than FCS.