الفهرس 
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Abstract
A total of 100 samples of (70 dog blood samples, 15 human blood samples and 15 hard ticks) were used to study the prevalence of Borrelia burgdorferi. Blood films were examined for at least 100 oil immersion fields (OIFs), for detection of helically shaped Borellia burgdorferi. No spiral shape bacterium was found in any blood smear of the examined dog. Borre¬lia burgdorferi could not be recovered from all the given samples by culturing on BSK-H medium, while seroprevalence to B burgdorferi of 23% in canine patients, while seroprevelance in contact human were (20%). DNA was extracted from both blood samples of dogs and human and ticks for detection of Borrelia burgdorferi DNA by PCR. The results observed revealed that the gene of ospA (309 bp ) was present only in four extracted DNA from different samples(human blood ,dog blood and hard ticks ). Three dog’s samples were positive with prevalence to B burgdorferi of 4.2% in canine patients, only one tick sample was positive by PCR with 6.6% incidence in ticks. However, B burgdorferi not detected in contact human blood.