الفهرس 
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Abstract
1
The study included seventy five male patients presenting to the Andrology & STDs Department, Faculty of Medicine, Cairo University.
According to their semen analysis parameters, they were allocated into the following groups:
I- Healthy fertile control (n=20)
II- Infertile oligoasthenotertatozoospermic (OAT) men (n=55).
Exclusion criteria were; varicocele, smoking, leucocytospermia and hormonal therapy.
They were subjected to:
1- Complete history taking:
2- Clinical examination.
3- Semen analysis according to WHO (2010) guidelines.
4- Estimation of eNOS gene polymorphism (by PCR-RFLP).
The results were as follows:
1. Comparing fertile controls with infertile OAT men, eNOS genotype G894T demonstrated prevalence of 50% versus 36.8 % for wild type (GG) with significant difference (P=0.046), 47.5% versus 35.0% for heterozygous type (GT) with significant difference (P=0.045) and 2.5% versus 28.2% for mutant homozygous type (TT) with significant difference (P=0.001).
2. eNOS genotype T786C demonstrated prevalence of 51.3% versus 37.3% for wild type (TT) with significant difference (P=0.043), 45% versus 32.7% for heterozygous type (TC) with significant difference (P=0.043) and 3.7% versus 30% for mutant type (CC) with significant difference (P=0.001).
3. Compared with GG homozygotes, carriers with the A allele exhibited more than a 2.846-fold increased risk of OAT occurrence in the OAT men compared the controls.
4. G894T genotype demonstrated significant decrease in G allele frequency (54.3% versus 74.4%, P=0.004) and significant increase in T allele frequency (45.7% versus 25.6%, P=0.003). T786C genotype demonstrated significant decrease in T allele frequency (53.6% versus 73.7%, P=0.002) and significant increase in C allele frequency (46.4% versus 26.4%, P=0.004).
5. Compared with TT homozygotes, carriers with the C allele exhibited more than a 1.772-fold increased risk of OAT occurrence.
6. G894T and T786C genotypes demonstrated significant negative correlation with sperm count (P=0.02; P=0.005), total sperm motility (P=0.001; P=0.001), sperm normal forms (P=0.008; P=0.001), seminal GPx (P=0.001; P=0.001) and significant positive correlation with seminal MDA (P=0.001; P=0.001). G894T genotype demonstrated significant positive correlation with T786C genotype (P=0.001).
It is concluded that there is a significant relationship between eNOS genotypes ”T-786C” ”G-894T” polymorphisms with decreased sperm parameters and increased seminal oxidative stress.