الفهرس 
Stem cellsOnly 14 pages are availabe for public view
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Abstract
Head and neck squamous cell carcinoma (HNSCC)
accounts for over 90% of all head and neck cancers. The
umbilical cord is the cable linking the fetus to the placenta. It
usually has two arteries and a vein, suspended in a hydrated
extracellular matrix known as Wharton’s Jelly. Wharton’s jelly
mesenchymal stem cells (WJMSCs) may be more useful for
cancer therapy than other adult stem cells, since they are
easily prepared in relatively large quantities from umbilical cord
after delivery and pose no ethical issues. L-amino acid
oxidases (LAAOs) are widely distributed in the venomous
snake families Viperidae, Crotalidae and Elapidae. Several
related proteins with LAAO activity have been described that
exhibit antimicrobial, antineoplastic or apoptosis inducing
activity. It is assumed that its general mechanism of toxicity is
based on the generation of cytotoxic amounts of H2O2.
This study was carried out to determine the anticancer
effects of WJMSCs conditioned medium (WJMSCs-CM), Lamino acid oxidase (LAAO) and the combination of WJMSCsCM and LAAO on HEP-2 cell line after 24, 48 and 72 hours.
Flowcytometry was used to detect the percentage of apoptosis
and real time PCR was used to detect the expression of p53
and Bcl-2 genes.
The results of the study showed that LAAO alone had
the greatest cytotoxic effect on HEP-2 cells followed by the combination between WJMSCs-CM and LAAO then WJMSCsCM alone.
from the results of this study it could be concluded that
the maximum effect of WJMSCs-CM and the combination on
HEP-2 cells was noted after 48 hours but, the maximum effect
of L-amino acid oxidase (LAAO) on HEP-2 cells was noted
after 72 hours.